Histone 3 Antikörper (H3K4me3)

Produktnummer ABIN6952351

Dieses Anti-Histone 3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Histone 3 in WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag, PeA und SNAP-ChIP. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für Histone 3 Antikörper (H3K4me3) (ABIN6952351)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Peptide Array (PeA), Sample Normalization and Antibody Profiling ChIP (SNAP-ChIP)

Güteklasse: ChIP-seq Grade

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K4me3

Spezifität: Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 4 (H3K4me3), using a KLH-conjugated synthetic peptide.

Kreuzreaktivität: Arabidopsis, Daphnia, Maize/Corn (Zea mays), Maus, Poplar (Populus), Reis (Oryza sativa), Tomate (Solanum lycopersicum), Forelle, Zebrafisch (Danio rerio)

Kreuzreaktivität (Details): wide range

Aufreinigung: Peptide affinity purified

Immunogen: synthetic peptide

Anwendungsinformationen

Applikationshinweise: ChIP/ChIP-seq<1 μg:ChIP
ELISA 1:1,000
Dot Blot 1:10,000
Peptde Array 1:10,000
WB 1:1,000
IF 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.3 μg/μL

Buffer: PBS, 0.05 % azide, 0.05 % ProClin300

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-Histone 3 Antikörper (ABIN6952351)

Schreiber, Mercier, Jiménez, Ye, García-Sánchez, Klein, Meunier, Ghimire, Birck, Jost, de Lichtenberg, Honoré, Serup, Gradwohl: "Extensive NEUROG3 occupancy in the human pancreatic endocrine gene regulatory network." in: Molecular metabolism, Vol. 53, pp. 101313, (2021) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone 3

Hintergrund: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K4 is associated with activation of gene transcription.

UniProt: P68431