Histone 3 Antikörper (H3K27ac)

Produktnummer ABIN6952337

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Histone 3 in WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag und PeA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Histone 3 Antikörper (H3K27ac) (ABIN6952337)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Peptide Array (PeA)

Güteklasse: ChIP-seq Grade

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K27ac

Spezifität: Polyclonal antibody raised in rabbit against the region of histone H3 containing the acetylated lysine 27 (H3K27ac), using a KLH-conjugated synthetic peptide.

Kreuzreaktivität: Arabidopsis, Maus, Ratte (Rattus)

Kreuzreaktivität (Details): wide range

Aufreinigung: Peptide affinity purified

Immunogen: synthetic peptide

Anwendungsinformationen

Applikationshinweise: ChIP/ChIP-seq 1 μg:ChIP
ELISA 1:500
Dot Blot 1:20,000
WB 1:1,000
IF 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 2.8 μg/μL

Buffer: PBS, 0.05 % azide, 0.05 % ProClin300

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone 3

Hintergrund: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H3K27 is associated with active promoters and enhancers.

UniProt: P68431