Topoisomerase I Antikörper

Produktnummer ABIN6945193

Der Kaninchen Monoklonal Anti-Topoisomerase I-Antikörper wurde für WB, IHC, FACS, IHC (p) und IF (cc) validiert. Er ist geeignet, Topoisomerase I in Proben von Human und Maus zu detektieren.

Kurzübersicht für Topoisomerase I Antikörper (ABIN6945193)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase I Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Klon: 1D1

Produktdetails anti-Topoisomerase I Antikörper

Kreuzreaktivität: Human, Maus

Aufreinigung: Purified by Protein A.

Immunogen: Human Topoisomerase I between 1-100 amino acids

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Andere Bezeichnung: Topoisomerase I

Substanzklasse: Viral Protein

Hintergrund:

Synonyms: DNA topoisomerase 1, DNA topoisomerase I, TOP1

Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

Gen-ID: 7150

UniProt: P11387

Pathways: Caspase Kaskade in der Apoptose, Stem Cell Maintenance