SAMHD1 Antikörper

Produktnummer ABIN6944815

Der Kaninchen Monoklonal Anti-SAMHD1-Antikörper wurde für WB, FACS, IF (cc), IF (p) und IHC (p) validiert. Er ist geeignet, SAMHD1 in Proben von Human zu detektieren.

Kurzübersicht für SAMHD1 Antikörper (ABIN6944815)

Target: SAMHD1 (SAM Domain and HD Domain 1 (SAMHD1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser SAMHD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 16C6

Produktdetails anti-SAMHD1 Antikörper

Kreuzreaktivität: Human

Aufreinigung: Purified by Protein A.

Immunogen: Synthetic peptide within human SAMHD1 aa 600 to the C-terminus

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu SAMHD1

Target: SAMHD1 (SAM Domain and HD Domain 1 (SAMHD1))

Andere Bezeichnung: SAMHD1

Hintergrund:

Synonyms: Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, Dendritic cell-derived IFNG-induced protein, Monocyte protein 5, SAM domain and HD domain-containing protein 1, dNTPase, DCIP, MOP-5, SAMHD1, MOP5.

Background: SAMHD1 may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome. SAMHD1 is a cellular enzyme, responsible for blocking replication of HIV in dendritic cells, macrophages and monocytes. It is an enzyme that exhibits phosphohydrolase activity, converting deoxynucleoside triphosphates (dNTPs) to inorganic phosphate (iPPP) and a 2'-deoxynucleoside (i.e. deoxynucleosides without a phosphate group). In doing so, SAMHD1 depletes the pool of dNTPs available to a reverse transcriptase for viral cDNA synthesis and thus prevents viral replication. SAMHD1 has also shown nuclease activity. Although a ribonuclease activity was described to be required for HIV-1 restriction, recent data confirmed that SAMHD1-mediated HIV-1 restriction in cells does not involve ribonuclease activity.

Gen-ID: 25939

UniProt: Q9Y3Z3