PP2A alpha/beta Antikörper

Produktnummer ABIN6944644

Der Kaninchen Monoklonal Anti-PP2A alpha/beta-Antikörper wurde für WB, IF (cc), IHC (p) und IHC validiert. Er ist geeignet, PP2A alpha/beta in Proben von Human, Maus, Ratte und Zebrafisch (Danio rerio) zu detektieren.

Kurzübersicht für PP2A alpha/beta Antikörper (ABIN6944644)

Target: PP2A alpha/beta (PP2A A/B)

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser PP2A alpha/beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Klon: 11F10

Produktdetails anti-PP2A alpha/beta Antikörper

Kreuzreaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio)

Aufreinigung: Purified by Protein A.

Immunogen: Human PP2A alpha + beta aa 250-350

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu PP2A alpha/beta

Target: PP2A alpha/beta (PP2A A/B)

Andere Bezeichnung: PP2A alpha + beta

Hintergrund:

Synonyms: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, Replication protein C, Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, PP2A-alpha, RP-C, PPP2CA, PP2A-beta, PPP2CB

Background: The catalytic subunit of protein phosphatase 2A (PP2A) is inactivated by in vitro phosphorylation of Tyr-307 by receptor and nonreceptor protein tyrosine kinases. The catalytic subunit of PP2A is phosphorylated by tyrosine-specific protein kinases and associates with a variety of regulatory subunits. Phosphorylation is enhanced in the presence of the phosphatase inhibitor okadaic acid, consistent with an autodephosphorylation reaction. Phosphorylation is catalyzed by p60v-src, p56lck, epidermal growth factor receptors, and insulin receptors. Transient deactivation of PP2A might enhance transmission of cellular signals through kinase cascades within cells. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases.

UniProt: P62714, P67775