IKK-alpha /IKK-beta Antikörper

Produktnummer ABIN6943783

Der Kaninchen Monoklonal anti-IKK-alpha /IKK-beta Antikörper wird verwendet zum Nachweis von IKK-alpha /IKK-beta in Proben von Maus, Human und Ratte. Er wurde validiert für WB, IF (cc) und IHC (p).

Kurzübersicht für IKK-alpha /IKK-beta Antikörper (ABIN6943783)

Target: IKK-alpha /IKK-beta

Reaktivität: Maus, Human, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser IKK-alpha /IKK-beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 4D4

Produktdetails anti-IKK-alpha /IKK-beta Antikörper

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: Recombinant human IKK alpha + IKK beta between 450 amino acids to C-terminus

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu IKK-alpha /IKK-beta

Target: IKK-alpha /IKK-beta

Andere Bezeichnung: IKK alpha + IKK beta

Hintergrund:

Synonyms: Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, Nuclear factor NF-kappa-B inhibitor kinase alpha, Transcription factor 16, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B kinase 2, Nuclear factor NF-kappa-B inhibitor kinase beta, I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, IKK1, NFKBIKA, TCF-16, CHUK, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, IKK2, NFKBIKB, IKBKB

Background: Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.

Gen-ID: 3551

UniProt: O14920