H2AFY Antikörper (AA 150-300)

Produktnummer ABIN6943601

Der Kaninchen Monoklonal anti-H2AFY Antikörper (Klon 6C8) (ABIN6943601) detektiert spezifisch H2AFY in WB, IHC (p), IF (cc) und IF (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für H2AFY Antikörper (AA 150-300) (ABIN6943601)

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser H2AFY Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Klon: 6C8

Produktdetails anti-H2AFY Antikörper

Bindungsspezifität: AA 150-300

Verwendungszweck: H2AFY (6C8) Monoclonal Antibody

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: Recombinant protein within human macroH2A.1 aa 150-300

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB(1:300-5000), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu H2AFY

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Andere Bezeichnung: H2AFY

Hintergrund:

Synonyms: macroH2A.1, Core histone macro-H2A.1, Histone H2A.y, Medulloblastoma antigen MU-MB-50.205, Histone macroH2A1, mH2A1, H2A/y, H2AFY, MACROH2A1, H2A histone family member Y.

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H2AFY is involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. H2AFY inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.

Gen-ID: 9555

UniProt: O75367