FTO Antikörper

Produktnummer ABIN6943480

Der Kaninchen Monoklonal Anti-FTO-Antikörper wurde für WB, IHC (p), IF (cc), IF (p) und FACS validiert. Er ist geeignet, FTO in Proben von Human zu detektieren.

Kurzübersicht für FTO Antikörper (ABIN6943480)

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser FTO Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Klon: 12A1

Produktdetails anti-FTO Antikörper

Kreuzreaktivität: Human

Aufreinigung: Purified by Protein A.

Immunogen: Recombinant protein within human FTO aa 350-500

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu FTO

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Andere Bezeichnung: FTO

Hintergrund:

Synonyms: Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein, FTO, KIAA1752, GDFD, ALKBH9, BMIQ14, Fatso.

Background: RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. M6A demethylation by FTO affects mRNA expression and stability. Also able to demethylate m6A in U6 small nuclear RNA (snRNA). Also acts as a tRNA demethylase by removing N1-methyladenine from various tRNAs. Also able to repair alkylated DNA and RNA by oxidative demethylation. Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation. Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells. Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs. Plays an oncogenic role in a number of acute myeloid leukemias by enhancing leukemic oncogene-mediated cell transformation: acts by mediating m6A demethylation of target transcripts such as MYC, CEBPA, ASB2 and RARA, leading to promote their expression

Gen-ID: 79068

UniProt: Q9C0B1