Cullin 4A Antikörper (AA 600-759)

Produktnummer ABIN6942738

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch Cullin 4A in WB, FACS, IHC (p), IF (cc) und IF (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Cullin 4A Antikörper (AA 600-759) (ABIN6942738)

Target: Cullin 4A (CUL4A)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Cullin 4A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Klon: 3C3

Produktdetails anti-Cullin 4A Antikörper

Bindungsspezifität: AA 600-759

Verwendungszweck: Cullin 4A (3C3) Monoclonal Antibody

Kreuzreaktivität: Human

Aufreinigung: Purified by Protein A.

Immunogen: Recombinant protein within human Cullin 4a aa 600-759

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB(1:300-5000), FCM(1:200-400), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Cullin 4A

Target: Cullin 4A (CUL4A)

Andere Bezeichnung: CUL4A

Hintergrund:

Synonyms: Cullin-4A, CUL-4A, CUL4A,

Background: CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins. CUL4A is a core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL.

Gen-ID: 8451

UniProt: Q13619