CHAF1B Antikörper

Produktnummer ABIN6942548

Produktdetails anti-CHAF1B Antikörper

Target: CHAF1B (Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CHAF1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Kreuzreaktivität: Human

Aufreinigung: Purified by Protein A.

Immunogen: Recombinant protein corresponding to the C-terminus of Human p60 CAF1.

Klon: 9A7

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
IP(1-2 μg)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu CHAF1B

Target: CHAF1B (Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))

Andere Bezeichnung: CHAF1B (CHAF1B Produkte)

Synonyme: CHAF1B antikoerper, wu:fd07c09 antikoerper, wu:fe36g06 antikoerper, wu:fv38g09 antikoerper, zgc:56096 antikoerper, CAF-1 antikoerper, CAF-IP60 antikoerper, CAF1 antikoerper, CAF1A antikoerper, CAF1P60 antikoerper, MPHOSPH7 antikoerper, MPP7 antikoerper, 2600017H24Rik antikoerper, C76145 antikoerper, CAF-I p60 antikoerper, CAF-Ip60 antikoerper, caf-1 antikoerper, CAF-1P60 antikoerper, chromatin assembly factor 1 subunit B antikoerper, chromatin assembly factor 1, subunit B antikoerper, chromatin assembly factor 1, subunit B (p60) antikoerper, chromatin assembly factor 1 subunit B L homeolog antikoerper, CHAF1B antikoerper, chaf1b antikoerper, Chaf1b antikoerper, chaf1b.L antikoerper

Hintergrund:

Synonyms: Chromatin assembly factor 1 subunit B, Chromatin assembly factor I p60 subunit, M-phase phosphoprotein 7, CAF-1 subunit B, CAF-I 60 kDa subunit, CAF-I p60, CHAF1B, CAF1A, CAF1P60, MPHOSPH7, MPP7, p60 CAF1

Background: Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin-associated proteins 1 (HP1_, HP1_ and HP1_). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Gen-ID: 8208

UniProt: Q13112