Rekombinanter BUB3 Antikörper (N-Term)

Produktnummer ABIN6942297

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch BUB3 in WB, FACS, IHC (p), IF (cc), IF (p) und IP. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter BUB3 Antikörper (N-Term) (ABIN6942297)

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser BUB3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunoprecipitation (IP)

Produktdetails anti-BUB3 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: BUB3 Recombinant Antibody

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: Recombinant protein corresponding to the N-terminus of human Bub3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200), IP(1-2 μg)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu BUB3

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Andere Bezeichnung: Bub3

Hintergrund:

Synonyms: Mitotic checkpoint protein BUB3, BUB3

Background: Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

Gen-ID: 9184

UniProt: O43684

Pathways: Maintenance of Protein Location