Rekombinanter Biotin Antikörper
Kurzübersicht für Rekombinanter Biotin Antikörper (ABIN6941455)
Target
Antikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Spezifität
- It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
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Immunogen
- Biotinylated sheep immunoglobulin
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Isotyp
- IgG
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anti-Biotin antibody
WB, ELISA, IHC, FACS, IF, FM Wirt: Maus Monoclonal 4C7-D5 unconjugated
anti-Biotin antibodyWB, IHC, FACS, IF, ISt, CM Wirt: Maus Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyWB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated
anti-Biotin antibody (DyLight 488)WB, FACS, IF, FM, FLISA, DB, MA Wirt: Ziege Polyclonal DyLight 488
anti-Biotin antibodyELISA, FACS Wirt: Maus Monoclonal SB58c unconjugated
anti-Biotin antibodyELISA, FACS, IP, MS Wirt: Maus Monoclonal 1D4-C5 unconjugated
anti-Biotin antibodyReaktivität: Human WB Wirt: Kaninchen Monoclonal unconjugated
anti-Biotin antibodyWB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated
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Applikationshinweise
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Positive Control: Biotinylated proteins in solution or on tissues.
Known Application: In situ Nucleic Acid Hybridization, Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)Optimal dilution for a specific application should be determined.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 200 μg/mL
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Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Haltbarkeit
- 24 months
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- Biotin
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Andere Bezeichnung
- Biotin (Vitamin B7 or Vitamin H)
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Substanzklasse
- Chemical
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Molekulargewicht
- 244Da
Target
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