MLANA & SILV Antikörper

Produktnummer ABIN6941419

Der Maus Monoklonal anti-MLANA & SILV Antikörper (Klon DT101-BC199-HMB45) (ABIN6941419) detektiert spezifisch MLANA & SILV in IHC, ISt und StM. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für MLANA & SILV Antikörper (ABIN6941419)

Target: MLANA & SILV

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Klon: DT101-BC199-HMB45

Produktdetails

Spezifität: This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant hMART-1 protein (DT101 & BC199), Extract of pigmented melanoma metastases from lymph nodes (HMB45)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: MLANA & SILV

Molekulargewicht: 20-22kDa (doublet) (MART-1): 90-100kDa (gp100)

Gen-ID: 2315, 6490

UniProt: Q16655, P40967