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Keratin 5,6,18 Antikörper

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in IHC, StM, WB, FACS und IF. Er zeigt eine Reaktivität gegenüber Human, Ratte und Rind (Kuh).
Produktnummer ABIN6941418

Kurzübersicht für Keratin 5,6,18 Antikörper (ABIN6941418)

Target

Keratin 5,6,18

Reaktivität

Human, Ratte, Rind (Kuh)

Wirt

  • 4
Maus

Klonalität

  • 4
Monoklonal

Konjugat

  • 4
Unkonjugiert

Applikation

  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon

SPM267
  • Spezifität

    It recognizes polypeptides of 58 kDa, 56 kDa, and 45 kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.

    Aufreinigung

    Purified by Protein A/G

    Immunogen

    Detergent-insoluble fraction of psoriatic human epidermis

    Isotyp

    IgG1 kappa
  • Applikationshinweise

    Positive Control: HeLa, HepG2 or PC12 cells. Tonsil or Skin.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Haltbarkeit

    24 months
  • Target

    Keratin 5,6,18

    Andere Bezeichnung

    Cytokeratin 5/6/18

    Molekulargewicht

    45-58kDa
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