Rekombinanter Cytokeratin, Multi (Epithelial Marker) Antikörper

Produktnummer ABIN6941405

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in IHC und StM. Er zeigt eine Reaktivität gegenüber Rind (Kuh), Frosch, Ziege, Meerschweinchen, Human, Affe, Maus, Schwein und Ratte.

Kurzübersicht für Rekombinanter Cytokeratin, Multi (Epithelial Marker) Antikörper (ABIN6941405)

Target: Cytokeratin, Multi (Epithelial Marker)

Antikörpertyp: Recombinant Antibody

Reaktivität: Rind (Kuh), Frosch, Ziege, Meerschweinchen, Human, Affe, Maus, Schwein, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Klon: RKRT-457

Produktdetails

Spezifität: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Aufreinigung: Purified by Protein A/G

Immunogen: Keratin-enriched preparation from cultured human epithelial cells

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: A431 cells, Skin, Colon carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: Cytokeratin, Multi (Epithelial Marker)

Gen-ID: 3851, 3852, 3853

UniProt: P02538, P04259, P13647, P19013, P48668