Melanoma Associated Antigen (KBA.62) Antikörper

Produktnummer ABIN6941354

Der Maus Monoklonal anti-Melanoma Associated Antigen (KBA.62) Antikörper (Klon KBA-62) (ABIN6941354) detektiert spezifisch Melanoma Associated Antigen (KBA.62) in IF und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Melanoma Associated Antigen (KBA.62) Antikörper (ABIN6941354)

Target: Melanoma Associated Antigen (KBA.62)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (IHC)

Klon: KBA-62

Produktdetails

Verwendungszweck: KBA.62 (Melanoma Associated Antigen) Antibody

Spezifität: KBA.62 is a novel anti-melanoma antibody. It reacts positively against melanocytic tumors but not other tumors, thus demonstrating specificity and sensitivity. Moreover, it reacts positively against junctional nevus cells but not intradermal nevi, and against fetal melanocytes but not normal adult melanocytes. KBA.62 antibody is useful in identifying malignant melanomas. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, sarcomas, spindle cell carcinomas and various types of mesenchymal neoplasms. A keratin-negative, vimentin-rich neoplasm that immuno-reacts with antibody to S100 protein and with KBA.62 antibody is, with rare exception, a melanoma. Anti-KBA.62 is a useful additional marker for melanoma, specifically in desmoplastic/spindle cell cases and in the context of micro-metastasis in sentinel lymph node.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human KAL cells derived from lymph node metastasis of malignant melanoma

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Known Application: Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: Melanoma Associated Antigen (KBA.62)

Andere Bezeichnung: KBA.62 (Melanoma Associated Antigen)

Hintergrund:

Human Melanoma Associated Antigen, KBA.62

SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Molekulargewicht: 135 and 128kDa and two weak bands of 88 and 73kDa)| (140