Myeloid Cell Marker (Macrophage / Granulocyte Marker) Antikörper

Produktnummer ABIN6941348

Produktdetails

Target: Myeloid Cell Marker (Macrophage / Granulocyte Marker)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Staining Methods (StM), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunostaining (ISt), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: Recognizes 183 kDa protein with DNA-binding characteristics, which is identified as a myeloid specific antigen. It reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. This MAb reacts with early precursor and mature forms of human myeloid cells. It is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of this antigen is not clear, although it has been proposed that it may play a role in the differentiation of myeloid cells.

Aufreinigung: Purified by Protein A/G

Immunogen: Human peripheral blood mononuclear cells

Klon: BM-1

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HL60 cells. Bone marrow, lymph node or tonsil.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (No special pretreatment is required for staining of formalin/paraffin tissues.)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: Myeloid Cell Marker (Macrophage / Granulocyte Marker)

Molekulargewicht: 183kDa