CDC34 Antikörper

Produktnummer ABIN6941292

Dieser Maus Monoklonal Antikörper detektiert spezifisch CDC34 in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CDC34 Antikörper (ABIN6941292)

Target: CDC34 (Cell Division Cycle 34 (CDC34))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDC34 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: CPTC-CDC34-2

Produktdetails anti-CDC34 Antikörper

Verwendungszweck: Cell Division Cycle 34 homolog Antibody

Spezifität: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitinconjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphasepromoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length CDC34 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: 293, K562, Jurkat cell lysates. Human small intestine.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CDC34

Target: CDC34 (Cell Division Cycle 34 (CDC34))

Andere Bezeichnung: CDC34

Hintergrund:

Cdc34, cell division cycle 34, UBC3, UBCH3, UBE2R1, Ubiquitin carrier protein, Ubiquitin conjugating enzyme E2 32 kDa complementing, Ubiquitin protein ligase R1, Ubiquitin conjugating enzyme E2-CDC34

Jurkat cell lysates. Human small intestine., K562, A-549 and HEK293

Molekulargewicht: 34kDa

Gen-ID: 997, 514997

UniProt: P49427