Rekombinanter MVP Antikörper

Produktnummer ABIN6941288

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch MVP in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter MVP Antikörper (ABIN6941288)

Target: MVP (Major Vault Protein (MVP))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MVP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: VP2897R

Produktdetails anti-MVP Antikörper

Verwendungszweck: Recombinant Major Vault Protein (MVP) Antibody

Spezifität: Recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for 70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human MVP protein

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7/HeLa, breast tumors.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MVP

Target: MVP (Major Vault Protein (MVP))

Andere Bezeichnung: MVP

Hintergrund:

Lung resistance-related protein (LRP), Major Vault Protein, MVP, VAULT1

A549, MCF-7 or HeLa cells. Breast tumors, Human Heart, Human Liver, Human Lung, Human Spleen

Molekulargewicht: 104-110kDa

Gen-ID: 9961, 632177

UniProt: Q14764