Rekombinanter CD34 Antikörper

Produktnummer ABIN6941175

Dieses Anti-CD34-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von CD34 in FACS, IHC und StM. Geeignet für Human und Ratte.

Kurzübersicht für Rekombinanter CD34 Antikörper (ABIN6941175)

Target: CD34

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD34 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)

Klon: HPCA1-2598R

Produktdetails anti-CD34 Antikörper

Spezifität: This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85 % of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human HPCA1 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: Jurkat, KG-1 cells. Tonsil or Angiosarcoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD34

Target: CD34

Andere Bezeichnung: CD34

Molekulargewicht: 90-110kDa

Gen-ID: 947

UniProt: P28906