CD5 Antikörper (AA 450-495)

Produktnummer ABIN6941052

Dieser Maus Monoklonal Antikörper detektiert spezifisch CD5 in FACS, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Rind (Kuh), Kaninchen, Pferd, Schwein, Affe, Huhn und Meerschweinchen.

Kurzübersicht für CD5 Antikörper (AA 450-495) (ABIN6941052)

Target: CD5

Reaktivität: Human, Ratte, Rind (Kuh), Kaninchen, Pferd, Schwein, Affe, Huhn, Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD5 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: CD5-54-F6

Produktdetails anti-CD5 Antikörper

Bindungsspezifität: AA 450-495

Verwendungszweck: CD5 (Mantle Cell Lymphoma Marker) Antibody

Spezifität: Recognizes a 67 kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa450-495) of human CD5 protein (intracellular region).

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: 293T, Ramos or MOLT-4 cells. Tonsil.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD5

Target: CD5

Andere Bezeichnung: CD5

Hintergrund:

CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5

293T, Ramos or MOLT-4 cells. Human tonsil.

Molekulargewicht: ~67kDa

Gen-ID: 921, 58685

UniProt: P06127