PDLIM1 Antikörper

Produktnummer ABIN6941009

Dieses Anti-PDLIM1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von PDLIM1 in WB, IHC und StM. Geeignet für Human.

Kurzübersicht für PDLIM1 Antikörper (ABIN6941009)

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PDLIM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Klon: CPTC-PDLIM1-1

Produktdetails anti-PDLIM1 Antikörper

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human full-length PDLIM1 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HepG2 cells and extracts from Jurkat. Human heart, colon or spleen.

Known Application: Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu PDLIM1

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Andere Bezeichnung: PDLIM1

Hintergrund: CLP36, also known as PDLIM1, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (-actinin-1, thereby facilitating the association of Clik1 with actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88 % sequence similarity with its rat counterpart, suggesting a conserved function between species.

Molekulargewicht: 36kDa

Gen-ID: 9124

UniProt: O00151