p63 Antikörper (AA 5-17)

Produktnummer ABIN6940969

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch p63 in WB, IHC und StM. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Rind (Kuh).

Kurzübersicht für p63 Antikörper (AA 5-17) (ABIN6940969)

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p63 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Produktdetails anti-p63 Antikörper

Bindungsspezifität: AA 5-17

Immunogen: A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: HEK293 cells. Prostate Carcinoma or Lung Squamous Cell Carcinoma.

Known Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu p63

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Andere Bezeichnung: TP63

Hintergrund: P40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.

Molekulargewicht: 40kDa

Gen-ID: 8626

UniProt: Q9H3D4