Calpastatin Antikörper

Produktnummer ABIN6940960

Produktdetails anti-Calpastatin Antikörper

Target: Calpastatin (CAST)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Calpastatin Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human CAST protein

Klon: CAST-1550

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells. Esophagus or Small Intestine.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Calpastatin

Target: Calpastatin (CAST)

Andere Bezeichnung: CAST (CAST Produkte)

Synonyme: BS-17 antikoerper, calp3 antikoerper, CAST antikoerper, sb:cb966 antikoerper, zgc:194249 antikoerper, cast antikoerper, DKFZp469E0716 antikoerper, calpastatin antikoerper, calpastatin S homeolog antikoerper, Calpastatin antikoerper, CAST antikoerper, Cast antikoerper, cast.S antikoerper, cast antikoerper, VDBG_09182 antikoerper, ical antikoerper

Hintergrund: Calpastatin is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpastatin, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpastatin co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.

Molekulargewicht: 126kDa

Gen-ID: 831

UniProt: P20810