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CA9 Antikörper

Dieses Anti-CA9-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CA9 in WB, IHC, IF, FACS und StM. Geeignet für Human und Pferd.
Produktnummer ABIN6940921

Kurzübersicht für CA9 Antikörper (ABIN6940921)

Target

Alle CA9 Antikörper anzeigen
CA9 (Carbonic Anhydrase IX (CA9))

Reaktivität

  • 96
  • 30
  • 23
  • 13
  • 5
  • 4
  • 4
  • 2
Human, Pferd

Wirt

  • 85
  • 37
Maus

Klonalität

  • 69
  • 53
Monoklonal

Konjugat

  • 64
  • 12
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser CA9 Antikörper ist unkonjugiert

Applikation

  • 85
  • 43
  • 33
  • 31
  • 25
  • 19
  • 17
  • 14
  • 13
  • 11
  • 10
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon

PN-15
  • Spezifität

    Recognizes a glycoprotein of ~200 kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

    Aufreinigung

    Purified by Protein A/G

    Immunogen

    Microsomal fraction of human renal cortical tissue homogenate

    Isotyp

    IgG2b kappa
  • Applikationshinweise

    Positive Control: Normal kidney or renal cell carcinoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Haltbarkeit

    24 months
  • Target

    CA9 (Carbonic Anhydrase IX (CA9))

    Andere Bezeichnung

    CA9

    Molekulargewicht

    55kDa

    Gen-ID

    768

    UniProt

    Q16790
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