WT1 Antikörper
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- Target Alle WT1 Antikörper anzeigen
- WT1 (Wilms Tumor 1 (WT1))
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Reaktivität
- Human, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser WT1 Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC), Staining Methods (StM)
- Spezifität
- Recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
- Aufreinigung
- Purified by Protein A/G
- Immunogen
- Recombinant human WT1 protein
- Isotyp
- IgG
- Top Product
- Discover our top product WT1 Primärantikörper
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- Applikationshinweise
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Positive Control: K562 cells. Wilm's Tumor, mesothelioma or fetal kidney.
Known Application: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Haltbarkeit
- 24 months
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- Target
- WT1 (Wilms Tumor 1 (WT1))
- Andere Bezeichnung
- Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker) (WT1 Produkte)
- Molekulargewicht
- 47-55kDa
- Gen-ID
- 7490
- UniProt
- P19544
- Pathways
- Tube Formation
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