WT1 Antikörper (AA 1-181)

Produktnummer ABIN6940900

Dieses Maus Monoklonal-Antikörper erkennt spezifisch WT1 in IHC und StM. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für WT1 Antikörper (AA 1-181) (ABIN6940900)

Target: WT1 (Wilms Tumor 1 (WT1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser WT1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Klon: 6F-H2

Produktdetails anti-WT1 Antikörper

Bindungsspezifität: AA 1-181

Spezifität: Recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm's tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm's tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm's tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant protein corresponding to residues 1-181 of human WT1.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: K562 cells. Kidney, Testis, Wilm's Tumor or Mesothelioma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Andere Bezeichnung: WT1

Molekulargewicht: 47-55kDa

Gen-ID: 7490

UniProt: P19544

Pathways: Tube Formation