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Vimentin Antikörper (AA 2-466)

Name: Vimentin Antikörper (AA 2-466)
Brand: antibodies-online
SKU: ABIN6940884
Price: 617.42 EUR
Availability: InStock

Dieses Anti-Vimentin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Vimentin in IHC und StM. Geeignet für Human.

Western Blot Analysis of U87 cell lysate using Vimentin Mouse Monoclonal Antibody (VIM/3736). (Vimentin Antikörper (AA 2-466))

Analysis of Protein Array containing more than 21,000 full-length human proteins using Vimentin Mouse Monoclonal Antibody (VIM/3736) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (Vimentin Antikörper (AA 2-466))

PAr

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Vimentin Mouse Monoclonal Antibody (VIM/3736). (Vimentin Antikörper (AA 2-466))

IHC

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Vimentin Mouse Monoclonal Antibody (VIM/3736). (Vimentin Antikörper (AA 2-466))

IHC

SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (VIM/3736). (Vimentin Antikörper (AA 2-466))

5 Abbildungen

Produktnummer ABIN6940884

Datenblatt (PDF)

Kurzübersicht für Vimentin Antikörper (AA 2-466) (ABIN6940884)

Target

Alle Vimentin (VIM) Antikörper anzeigen

Vimentin (VIM)

Reaktivität

Human

Wirt

Maus

Klonalität

Monoklonal

Konjugat

Dieser Vimentin Antikörper ist unkonjugiert

Applikation

Immunohistochemistry (IHC), Staining Methods (StM)

Klon

VIM-3736

Discover our top product VIM Primärantikörper

Bindungsspezifität
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AA 2-466

Spezifität

This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Keine Kreuzreaktivität

Maus, Ratte (Rattus)

Aufreinigung

Purified by Protein A/G

Immunogen

A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)

Isotyp

IgG2b kappa
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Affe WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reaktivität: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reaktivität: Human, Maus, Ratte WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human, Maus, Ratte, Schwein, Huhn WB, IHC (p), ICC, IP Wirt: Maus Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Säugetier WB, FACS, IHC (p), ICC Wirt: Maus Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human, Maus WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human WB, IHC, IF Wirt: Maus Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reaktivität: Human WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reaktivität: Human WB, IHC, IF, IP Wirt: Kaninchen Monoclonal unconjugated

Applikationshinweise

Positive Control: U87, A375, Raji, HEP2 cells. Sarcomas or Melanomas.
Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-80 °C

Informationen zur Lagerung

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit

24 months
Target

Vimentin (VIM)

Andere Bezeichnung

VIM

Molekulargewicht

57-60kDa

Gen-ID

7431

UniProt

P08670

Pathways

Caspase Kaskade in der Apoptose