TYR Antikörper
Kurzübersicht für TYR Antikörper (ABIN6940824)
Target
Alle TYR Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Spezifität
- Recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.
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Aufreinigung
- Purified by Protein A/G
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Immunogen
- Recombinant tyrosinase protein
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Isotyp
- IgG2a kappa
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Applikationshinweise
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Positive Control: SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells. Melanoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, or 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 200 μg/mL
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Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Haltbarkeit
- 24 months
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- TYR (Tyrosinase (TYR))
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Andere Bezeichnung
- TYR
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Molekulargewicht
- 70-80kDa
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Gen-ID
- 7299
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UniProt
- P14679
Target
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