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TYR Antikörper

Dieses Anti-TYR-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von TYR in IF, IHC, FACS und StM. Geeignet für Human.
Produktnummer ABIN6940824

Kurzübersicht für TYR Antikörper (ABIN6940824)

Target

Alle TYR Antikörper anzeigen
TYR (Tyrosinase (TYR))

Reaktivität

  • 100
  • 24
  • 23
  • 6
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Human

Wirt

  • 72
  • 37
Maus

Klonalität

  • 60
  • 50
Monoklonal

Konjugat

  • 65
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser TYR Antikörper ist unkonjugiert

Applikation

  • 61
  • 40
  • 32
  • 29
  • 27
  • 20
  • 19
  • 18
  • 14
  • 13
  • 13
  • 9
  • 3
  • 3
  • 1
  • 1
  • 1
Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Klon

T311
  • Spezifität

    Recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.

    Aufreinigung

    Purified by Protein A/G

    Immunogen

    Recombinant tyrosinase protein

    Isotyp

    IgG2a kappa
  • Applikationshinweise

    Positive Control: SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells. Melanoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, or 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Haltbarkeit

    24 months
  • Target

    TYR (Tyrosinase (TYR))

    Andere Bezeichnung

    TYR

    Molekulargewicht

    70-80kDa

    Gen-ID

    7299

    UniProt

    P14679
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