Rekombinanter TYMS Antikörper (AA 60-174)

Produktnummer ABIN6940823

Dieses Anti-TYMS-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von TYMS in IF, FACS, ELISA, RIA und Coat. Geeignet für Human.

Kurzübersicht für Rekombinanter TYMS Antikörper (AA 60-174) (ABIN6940823)

Target: TYMS (Thymidylate Synthetase (TYMS))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TYMS Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Radioimmunoassay (RIA), Coating (Coat)

Klon: RTYMS-1884

Produktdetails anti-TYMS Antikörper

Bindungsspezifität: AA 60-174

Spezifität: It recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MOLT4, Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Andere Bezeichnung: TYMS

Molekulargewicht: 36kDa

Gen-ID: 7298

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases