TIMP2 Antikörper (N-Term)

Produktnummer ABIN6940735

Der Maus Monoklonal anti-TIMP2 Antikörper (Klon 3A4) (ABIN6940735) detektiert spezifisch TIMP2 in WB, IHC, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Rind (Kuh), Kaninchen, Zebrafisch (Danio rerio) und Meerschweinchen.

Kurzübersicht für TIMP2 Antikörper (N-Term) (ABIN6940735)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Kaninchen, Zebrafisch (Danio rerio), Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TIMP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 3A4

Produktdetails anti-TIMP2 Antikörper

Bindungsspezifität: AA 56-66, N-Term

Verwendungszweck: TIMP2 (Tissue Inhibitor of Metalloproteinase 2) Antibody

Spezifität: It recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Andere Bezeichnung: TIMP2

Hintergrund:

Metalloproteinase inhibitor 2, TIMP metallopeptidase inhibitor 2, TIMP-2, TIMP2, Tissue Inhibitor of Metalloproteinase 2

Colon, Endometrial, HeLa cells. Normal Placenta. Breast, Prostate or Ovarian Carcinoma.

Molekulargewicht: 21kDa

Gen-ID: 7077, 633514

UniProt: P16035

Pathways: cAMP Metabolic Process