Transgelin Antikörper

Produktnummer ABIN6940678

Der Maus Monoklonal anti-Transgelin Antikörper (Klon TAGLN-247) (ABIN6940678) detektiert spezifisch Transgelin in IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Kaninchen, Rind (Kuh) und Schwein.

Kurzübersicht für Transgelin Antikörper (ABIN6940678)

Target: Transgelin (TAGLN)

Reaktivität: Human, Maus, Kaninchen, Rind (Kuh), Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transgelin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: TAGLN-247

Produktdetails anti-Transgelin Antikörper

Verwendungszweck: Transgelin (SM22-alpha) Antibody

Spezifität: This MAb recognizes a 22 kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human transgelin (TAGLN) protein.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Transgelin

Target: Transgelin (TAGLN)

Andere Bezeichnung: TAGLN

Hintergrund:

2 kDa actin-binding protein, Human 22 kDa smooth muscle protein, Protein WS3-10, SMCC, Smooth muscle protein 22-alpha (SM22-alpha), TAGLN, TAGLN1, transgelin, Transgelin variant 2, WS3-10

Hs68 or WI 38 cells. Colon carcinoma., U-2 OS

Molekulargewicht: 22kDa

Gen-ID: 6876, 410977

UniProt: Q01995