CD43 Antikörper

Produktnummer ABIN6940634

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD43 in FACS, WB, IHC, IF und StM. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD43 Antikörper (ABIN6940634)

Target: CD43 (SPN) (Sialophorin (SPN))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD43 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Klon: DF-T1

Produktdetails anti-CD43 Antikörper

Spezifität: It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. Epitope of MAb Bra7G is clearly different from that of MAb DF-T1, called b as opposed to a for DF-T1. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Aufreinigung: Purified by Protein A/G

Immunogen: Myeloblastic KG1 cells

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: PBMCs or K562 cells (FC). K562 cell lysate (WB). Paracortex in a tonsil or a reactive lymph node (IHC).

Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Andere Bezeichnung: SPN

Molekulargewicht: 95, 115, or 135kDa

Gen-ID: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity