SOX2 Antikörper (AA 176-305)

Produktnummer ABIN6940611

Dieses Anti-SOX2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von SOX2 in WB, ELISA, IHC, Coat und StM. Geeignet für Human und Maus.

Kurzübersicht für SOX2 Antikörper (AA 176-305) (ABIN6940611)

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SOX2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)

Klon: SOX2-1791

Produktdetails anti-SOX2 Antikörper

Bindungsspezifität: AA 176-305

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: A549, NCCIT cells. Lung or Cervicalcarcinoma.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Andere Bezeichnung: SOX2

Hintergrund: SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Molekulargewicht: 34kDa

Gen-ID: 6657

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis