Fascin Antikörper

Produktnummer ABIN6940609

Der Maus Monoklonal Anti-Fascin-Antikörper wurde für WB, IHC, FACS, IF und StM validiert. Er ist geeignet, Fascin in Proben von Human und Ratte zu detektieren.

Kurzübersicht für Fascin Antikörper (ABIN6940609)

Target: Fascin (FSCN1)

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fascin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Klon: FSCN1-418

Produktdetails anti-Fascin Antikörper

Spezifität: Recognizes a protein of 55 kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.

Aufreinigung: Purified by Protein A/G

Immunogen: Full length recombinant human FSCN1 protein

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: K562, HeLa, MCF-7, PC-3 or BEWO cells. Hodgkin's lymphoma, Ovarian or Testicular Carcinoma.

Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Fascin

Target: Fascin (FSCN1)

Andere Bezeichnung: FSCN1

Molekulargewicht: 55kDa

Gen-ID: 6624

UniProt: Q16658