SUMO2 Antikörper

Produktnummer ABIN6940603

Dieser Maus Monoklonal Antikörper detektiert spezifisch SUMO2 in IHC, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human und Ratte.

Kurzübersicht für SUMO2 Antikörper (ABIN6940603)

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SUMO2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: SUMO2-1199

Produktdetails anti-SUMO2 Antikörper

Verwendungszweck: SUMO-2 Antibody

Spezifität: The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include MDM2, p53, PML and RanGap1. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1 and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer's disease.

Kreuzreaktivität (Details): Predicted to show a broad species reactivity.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human SUMO2 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells. Breast carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu SUMO2

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Andere Bezeichnung: SUMO2

Hintergrund:

HSMT3, Sentrin 2, Small ubiquitin like modifier 2, Small ubiquitin like modifier protein 3, SMT3A, SMT3B, SMT3 homolog 1 (SMT3H1), SMT3 homolog 2 (SMT3H2), SMT3 homolog, SMT3 suppressor of mif two 3 homolog 1, SMT3 suppressor of mif two 3 homolog 2, SMT3 suppressor of mif two 3 homolog 3, Suppressor of mif two 3 homolog 2, Suppressor of mif two 3 homolog 3, Ubiquitin like protein SMT3A, Ubiquitin like protein SMT3B

HeLa cells. Breast carcinoma.

Molekulargewicht: 11-13kDa

Gen-ID: 6613, 474005

UniProt: P55854

Pathways: Methionine Biosynthetic Process