Rekombinanter s100a4 Antikörper (AA 1-200)

Produktnummer ABIN6940545

Der Kaninchen Monoklonal Anti-s100a4-Antikörper wurde für WB, IHC, IF, FACS und StM validiert. Er ist geeignet, s100a4 in Proben von Human und Maus zu detektieren.

Kurzübersicht für Rekombinanter s100a4 Antikörper (AA 1-200) (ABIN6940545)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser s100a4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon: S100A4-2750R

Produktdetails anti-s100a4 Antikörper

Bindungsspezifität: AA 1-200

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant fragment of human S100A4 protein (around aa1-200) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa, A549 or A375 cells. Placenta.

Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Andere Bezeichnung: S100A4

Hintergrund: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Molekulargewicht: 10-12kDa

Gen-ID: 6275

UniProt: P26447

Pathways: S100 Proteine