MAML3 Antikörper

Produktnummer ABIN6940389

Der Maus Monoklonal Anti-MAML3-Antikörper wurde für ISt, IHC und StM validiert. Er ist geeignet, MAML3 in Proben von Human zu detektieren.

Kurzübersicht für MAML3 Antikörper (ABIN6940389)

Target: MAML3 (Mastermind-Like 3 (MAML3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAML3 Antikörper ist unkonjugiert

Applikation: Immunostaining (ISt), Immunohistochemistry (IHC), Staining Methods (StM)

Klon: MAML3-1303

Produktdetails anti-MAML3 Antikörper

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human MAML3 protein fragment

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7 cells. Pancreas or Placenta. Cervix or Colon Carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MAML3

Target: MAML3 (Mastermind-Like 3 (MAML3))

Andere Bezeichnung: MAML3

Hintergrund: MAML3 (mastermind-like protein 3) is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4.This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain of MAML3 is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, upregulation of MAML3 is thought to be involved in oncogenesis.

Molekulargewicht: 150-170kDa

Gen-ID: 55534

UniProt: Q96JK9