NME2 Antikörper

Produktnummer ABIN6940215

Der Maus Monoklonal anti-NME2 Antikörper wird verwendet zum Nachweis von NME2 in Proben von Human. Er wurde validiert für WB, IHC und StM.

Kurzübersicht für NME2 Antikörper (ABIN6940215)

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NME2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Klon: CPTC-NME2-2

Produktdetails anti-NME2 Antikörper

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human NME2 protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: PC3, A549, HeLa, Jurkat cells. Ubiquitously expressed in all tissues.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu NME2

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Andere Bezeichnung: NME2

Hintergrund: The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Molekulargewicht: 17kDa

Gen-ID: 4831

UniProt: P22392