Myogenin Antikörper (AA 30-224, AA 73-94, AA 138-158)

Produktnummer ABIN6940163

Dieses Anti-Myogenin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Myogenin in ELISA, IHC, Coat und StM. Geeignet für Human, Maus, Ratte, Schwein, Cat und Hund.

Kurzübersicht für Myogenin Antikörper (AA 30-224, AA 73-94, AA 138-158) (ABIN6940163)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reaktivität: Human, Maus, Ratte, Schwein, Cat, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Myogenin Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)

Klon: F5D

Produktdetails anti-Myogenin Antikörper

Bindungsspezifität: AA 30-224, AA 73-94, AA 138-158

Aufreinigung: Purified by Protein A/G

Immunogen: Rat myogenin peptide (aa 73-94) followed by rat myogenin recombinant fragment (aa30-224) (Epitope aa138-158)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.

Known Application: ELISA (For coating, order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Andere Bezeichnung: MYOG

Hintergrund: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80 % amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.

Molekulargewicht: 34kDa

Gen-ID: 4656

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development