MUC5AC Antikörper

Produktnummer ABIN6940136

Dieser Maus Monoklonal Antikörper detektiert spezifisch MUC5AC in IF, FACS und ELISA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Affe, Cat und Rind (Kuh).

Kurzübersicht für MUC5AC Antikörper (ABIN6940136)

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reaktivität: Human, Maus, Affe, Cat, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC5AC Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), ELISA

Klon: 2-11M1

Produktdetails anti-MUC5AC Antikörper

Verwendungszweck: MUC5AC (Mucin 5AC / Gastric Mucin) Antibody

Spezifität: Mucins are a group of high molecular weight glycoproteins consisting of a mucin core protein and O-linked carbohydrates. Mucin 6 carries GlcNAc±1,Üí4Gal≤,ÜíR structures, indicating that ±1, 4-N-acetylglucosaminyltransferase is important to the formation of the mucous glycoproteins in vivo. Mucin 5AC is a gel-forming mucin that is secreted from surface mucous cells. Glucocorticoid is required for the expression of Mucin 5AC mRNA and high doses of hydrocortisone suppresses its expression. Additionally, asthmatic fluid stimulates Mucin 5AC synthesis several-fold. The pro-inflammatory cytokines IL-6 and TNF± stimulate Mucin 5AC secretion and thus contribute to the upregulation of mucin by chronic inflammation. Expression of Mucin 5AC is retinoic acid (RA)- or retinol-dependent, and RA control of mucin genes is mediated by the retinoid acid receptor RAR± and, to a lesser extent, by RAR≥. Thyroid hormone binding to thyroid receptors inhibits Mucin 5AC gene expression. Mucin 5AC is also expressed in normal endocervical epithelium, small intestine, gastric cells (Lewis type 1) and gastric metaplasia and it is one of the major mucins in the ethmoid mucosa.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7 cells. Human colon or stomach (IHC).

Known Application: ELISA (Use at 2-4 μg/mL for coating), Western Blot (1-2 μg/mL), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MUC5AC

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Andere Bezeichnung: MUC5AC

Hintergrund:

Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)

MCF-7 cells. Human stomach.

Molekulargewicht: 400-600 kDa

Gen-ID: 4586, 534332

UniProt: P98088