MUC3A Antikörper

Produktnummer ABIN6940123

Der Maus Monoklonal Anti-MUC3A-Antikörper wurde für IHC, IF, FACS und StM validiert. Er ist geeignet, MUC3A in Proben von Human zu detektieren.

Kurzübersicht für MUC3A Antikörper (ABIN6940123)

Target: MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC3A Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon: MUC3-1154

Produktdetails anti-MUC3A Antikörper

Spezifität: It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.

Keine Kreuzreaktivität: Ratte (Rattus)

Aufreinigung: Purified by Protein A/G

Immunogen: A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: LS174T cells. Human small intestine (IHC).

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MUC3A

Target: MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))

Andere Bezeichnung: MUC3A

Molekulargewicht: 1100kDa

Gen-ID: 4584, 57876

UniProt: Q02505, Q9H195