MUC1 Antikörper

Produktnummer ABIN6940109

Der Maus Monoklonal Anti-MUC1-Antikörper wurde für IHC und StM validiert. Er ist geeignet, MUC1 in Proben von Human zu detektieren.

Kurzübersicht für MUC1 Antikörper (ABIN6940109)

Target: MUC1 (Mucin 1 (MUC1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Klon: 115D8

Produktdetails anti-MUC1 Antikörper

Spezifität: This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. It is used as tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Its epitope is destroyed by desialylation i.e. treatment with Neuraminidase.

Aufreinigung: Purified by Protein A/G

Immunogen: Human milk-fat globule membranes (HMFGM)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MUC1

Target: MUC1 (Mucin 1 (MUC1))

Andere Bezeichnung: MUC1

Molekulargewicht: 265-400kDa

Gen-ID: 4582

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling