Rekombinanter MITF Antikörper

Produktnummer ABIN6940051

Der Kaninchen Monoklonal Anti-MITF-Antikörper wurde für IHC, IF, FACS und StM validiert. Er ist geeignet, MITF in Proben von Human und Hund zu detektieren.

Kurzübersicht für Rekombinanter MITF Antikörper (ABIN6940051)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Hund

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MITF Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon: MITF-2987R

Produktdetails anti-MITF Antikörper

Spezifität: MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This MAb recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human MiTF protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: Jurkat, A-431, HeLa or 501 Mel human melanoma cells. Melanoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Andere Bezeichnung: MITF

Molekulargewicht: 52-56kDa (doublet)

Gen-ID: 4286

UniProt: O75030

Pathways: Chromatin Binding