Rekombinanter EpCAM Antikörper (Extracellular Domain)

Produktnummer ABIN6939997

Der Kaninchen Monoklonal anti-EpCAM Antikörper wird verwendet zum Nachweis von EpCAM in Proben von Human. Er wurde validiert für FACS und IHC.

Kurzübersicht für Rekombinanter EpCAM Antikörper (Extracellular Domain) (ABIN6939997)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Klon: EGP40-2571R

Produktdetails anti-EpCAM Antikörper

Bindungsspezifität: AA 100-224, Extracellular Domain

Verwendungszweck: Recombinant Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody

Spezifität: Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Aufreinigung: 200ug/ml of Ab Purified by Protein A.

Immunogen: Recombinant human EpCAM fragment from extracellular domain (around aa100-224) (exact sequence is proprietary)

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7 or HT29 cells. Breast tumor.

Known Application: Flow Cytometry (1-2 μg/million cells). Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: TACSTD1

Hintergrund:

Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

MCF-7 or HT29 cells. Breast tumor.

Molekulargewicht: 40-43kDa

Gen-ID: 4072, 542050

UniProt: P16422