Rekombinanter EpCAM Antikörper (Extracellular Domain)

Produktnummer ABIN6939985

Der Maus Monoklonal anti-EpCAM Antikörper (Klon rEGP40-1372) (ABIN6939985) detektiert spezifisch EpCAM in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Hund und Cat.

Kurzübersicht für Rekombinanter EpCAM Antikörper (Extracellular Domain) (ABIN6939985)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Hund, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: REGP40-1372

Produktdetails anti-EpCAM Antikörper

Bindungsspezifität: AA 100-224, Extracellular Domain

Verwendungszweck: Recombinant Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody

Spezifität: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on basolateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100-224) (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HT29 cells. Ovarian carcinoma.

Known Application: Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: TACSTD1

Hintergrund:

Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1), ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, Membrane Component, Chromosome 17, Surface Marker-1 (M17S1), REG-1, Reggie-1, Reggie-2

MCF-7 or HT29 cells. Human ovarian carcinoma. Human Intestine, HEK293.

Molekulargewicht: 40-43kDa

Gen-ID: 4072, 542050

UniProt: P16422