EpCAM Antikörper (Extracellular Domain)
Quick Overview for EpCAM Antikörper (Extracellular Domain) (ABIN6939980)
Target
Alle EpCAM (EPCAM) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 77-202, Extracellular Domain
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Aufreinigung
- Purified by Protein A/G
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Immunogen
- Recombinant human EpCAM protein fragment from extracellular domain (around aa77-202) (exact sequence is proprietary)
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Isotyp
- IgG2b kappa
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Applikationshinweise
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Positive Control: MCF-7, HT29 cells, Lung or Ovarian carcinoma.
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 200 μg/mL
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Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Haltbarkeit
- 24 months
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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Andere Bezeichnung
- TACSTD1
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Hintergrund
- EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
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Molekulargewicht
- 40-43kDa
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Gen-ID
- 4072
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UniProt
- P16422
Target
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