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EpCAM Antikörper (Extracellular Domain)

Name: EpCAM Antikörper (Extracellular Domain)
Brand: antibodies-online
SKU: ABIN6939980
Price: 617.42 EUR
Availability: InStock

Der Maus Monoklonal Anti-EpCAM-Antikörper wurde für FACS, IF, IHC, ELISA, StM und Coat validiert. Er ist geeignet, EpCAM in Proben von Human zu detektieren.

Formalin-fixed, paraffin-embedded human Colorectal Carcinoma stained with Ep-CAM Mouse Monoclonal Antibody (EGP40/1798). (EpCAM Antikörper (Extracellular Domain))

IHC

Western Blot Analysis of MCF-7 cell lysate using Ep-CAM Monoclonal Antibody (EGP40/1798). (EpCAM Antikörper (Extracellular Domain))

Analysis of Protein Array containing >19,000 full-length human proteins using EpCAM Mouse Monoclonal Antibody (EGP40/1798) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (EpCAM Antikörper (Extracellular Domain))

PAr

Confocal Immunofluorescence of MCF-7 cells Ep-CAM Monoclonal Antibody (EGP40/1798) labeled with CF488 (Green); Reddot is used to label the nuclei. (EpCAM Antikörper (Extracellular Domain))

SDS-PAGE Analysis Purified EpCAM Mouse Monoclonal Antibody (EGP40/1798). (EpCAM Antikörper (Extracellular Domain))

SDS

5 Abbildungen

Produktnummer ABIN6939980

Kurzübersicht für EpCAM Antikörper (Extracellular Domain) (ABIN6939980)

Target

Alle EpCAM (EPCAM) Antikörper anzeigen

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reaktivität

Human

Wirt

Maus

Klonalität

Monoklonal

Konjugat

Dieser EpCAM Antikörper ist unkonjugiert

Applikation

Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Staining Methods (StM), Coating (Coat)

Klon

EGP40-1798

Discover our top product EPCAM Primärantikörper

Bindungsspezifität
91

55

33

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20

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8

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1

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1
AA 77-202, Extracellular Domain

Aufreinigung

Purified by Protein A/G

Immunogen

Recombinant human EpCAM protein fragment from extracellular domain (around aa77-202) (exact sequence is proprietary)

Isotyp

IgG2b kappa
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 40-260) antibody
EPCAM Reaktivität: Human WB, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 25-265) antibody
EPCAM Reaktivität: Schwein WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 53-150) antibody
EPCAM Reaktivität: Human, Maus, Ratte, Kaninchen WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) antibody
EPCAM Reaktivität: Human WB, FACS, IHC, ELISA Wirt: Maus Monoclonal 7B5C10 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 20-60), (N-Term) antibody
EPCAM Reaktivität: Human FACS, IHC, StM Wirt: Kaninchen Monoclonal EGP40-1555R unconjugated Recombinant Antibody

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM Reaktivität: Human WB, IHC, StM Wirt: Maus Monoclonal rEGP40-1372 unconjugated Recombinant Antibody

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM Reaktivität: Human FACS, IF, IHC, ELISA, StM, Coat Wirt: Maus Monoclonal EGP40-1384 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 280-350), (Cytoplasmic Domain) antibody
EPCAM Reaktivität: Human, Maus, Ratte IHC, StM Wirt: Maus Monoclonal EGP40-1110 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM Reaktivität: Human WB, FACS, IF, IHC, StM Wirt: Maus Monoclonal EGP40-1373 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM Reaktivität: Human WB, FACS, IF, IHC, StM Wirt: Maus Monoclonal EGP40-1372 unconjugated

Applikationshinweise

Positive Control: MCF-7, HT29 cells, Lung or Ovarian carcinoma.
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-80 °C

Informationen zur Lagerung

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit

24 months
Target

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung

TACSTD1

Hintergrund

EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Molekulargewicht

40-43kDa

Gen-ID

4072

UniProt

P16422