EpCAM Antikörper (Extracellular Domain)

Produktnummer ABIN6939977

Der Maus Monoklonal Anti-EpCAM-Antikörper wurde für WB, FACS, IF, IHC und StM validiert. Er ist geeignet, EpCAM in Proben von Human zu detektieren.

Kurzübersicht für EpCAM Antikörper (Extracellular Domain) (ABIN6939977)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Klon: EGP40-1372

Produktdetails anti-EpCAM Antikörper

Bindungsspezifität: AA 77-202, Extracellular Domain

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human EpCAM fragment from extracellular domain (around aa 77-202) (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7, HT29 cells. Ovarian carcinoma.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: TACSTD1

Hintergrund: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Molekulargewicht: 40-43kDa

Gen-ID: 4072

UniProt: P16422