Laminin gamma 1 Antikörper

Produktnummer ABIN6939955

Dieses Anti-Laminin gamma 1-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von Laminin gamma 1 in IHC, IF, FACS, ISt und StM. Geeignet für Human und Maus.

Kurzübersicht für Laminin gamma 1 Antikörper (ABIN6939955)

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Laminin gamma 1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM)

Klon: A5

Produktdetails anti-Laminin gamma 1 Antikörper

Spezifität: Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This MAb reacts with laminin B2/1 chain of ~210 kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific MAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This MAb stains uniformly all human and murine basement membranes.

Aufreinigung: Purified by Protein A/G

Immunogen: Murine EHS laminin preparation

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Normal colon or carcinomas.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Laminin gamma 1

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Andere Bezeichnung: LAMC1

Molekulargewicht: 210kDa

Gen-ID: 3915

UniProt: P11047, Q5VYE7, Q6NVY8