Rekombinanter Cytokeratin 19 Antikörper

Produktnummer ABIN6939940

Der Maus Monoklonal anti-Cytokeratin 19 Antikörper (Klon rKRT19-799) (ABIN6939940) detektiert spezifisch Cytokeratin 19 in WB, IHC, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter Cytokeratin 19 Antikörper (ABIN6939940)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 19 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: RKRT19-799

Produktdetails anti-Cytokeratin 19 Antikörper

Verwendungszweck: Recombinant Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker) Antibody

Spezifität: This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40 kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.

Keine Kreuzreaktivität: Frettchen, Ratte (Rattus)

Aufreinigung: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogen: Recombinant human full-length KRT19 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7, HeLa, Hep-G2 cells, Breast Cancer.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Andere Bezeichnung: KRT19

Hintergrund:

k19, k1cs, Keratin 19 Keratin Type i 40kD, krt19

HeLa, Hep-G2 cells. Human breast cancer tissue., MCF-7. Human Skin.

Molekulargewicht: 40kDa

Gen-ID: 3880, 654568

UniProt: P08727